Composite

Part:BBa_K3982006:Design

Designed by: Akankshya Ramkrishna Sahu   Group: iGEM21_IISER_Berhampur   (2021-10-01)


6xHis - Tagged Cas14a1 with TEV site


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1411
    Illegal BamHI site found at 1005
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1411
    Illegal PstI site found at 201
    Illegal PstI site found at 367
    Illegal AgeI site found at 661
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Restriction cloning using Xba1 and Spe1 RE.

Source

Addgene plasmid 112502

References